Antimicrobial, Antioxidant and Cytotoxic Activities of Different Fractions Obtained from Endophytic Fusarium solani F01 Isolated in Catharanthus roseus Collected in Vietnam

Abstract: Aim: Finding out the biological sources is essential for pharmaceutical field. Materials and Methods: Endophytic fungus was isolated from Catharanthus roseus originated in Vietnam then identified by comparing, phylogeny analyzing its 18S sRNA sequence using Blast search GenomeNet. The supernatant sonicated mycelia were fractionated with methanol, ethyl acetate, hexane. All fractions used to test antimicrobial, antioxidant, cytotoxicity activities. Antimicrobial activities on Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa 27853, Serratia marcescens 14756, Vibrio parahaemolyticus 17802, Escherichia coli 25922 performed well diffusion method. In terms of antioxidant activities, reducing power DPPH radical scavenging assay done. purification methanol extract performed. purified compounds activity. For activity, sulforhodamine B extracts breast cancer cell line (MCF-7). Results: Fusarium solani F01 identified. treated cell-free showed highest wider spectrum than acetate hexane extracts. Mycelial these solvents did not show except extract. crude gave higher (1.416 ± 0.067) ascorbic acid (0.577 0.01) while others very low at concentration (100 μg/ ml). Only obtained moderate activity when comparison their IC50 (9.17 g/ml) (1.28 g/ Four that but there one compound effects MCF-7 inhibition (80.34 1.28). Conclusion: study suggested could give many will be a potential source Key words: roseus, Identification, Molecular analysis, Biological Extraction, Purification.